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il 12p40  (R&D Systems)


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    Structured Review

    R&D Systems il 12p40
    Cytokine secretion assay of human iPSC-derived immature and mature dendritic cells. Interleukin (IL)-10 and <t>IL-12p40</t> secretion by human iPSC-derived immature dendritic cells after stimulation with LPS or co-culture with Lactobacillus rhamnosus GG. The “cell only” group refers to iPSC-derived immature dendritic cells without any stimulation. Plates were incubated for 24 h to measure cytokine secretion into the culture medium (*** p < 0.001). Data shown are representative of at least three independent differentiation batches.
    Il 12p40, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+23+duoset+elisa/Human+IL-12%2FIL-23+p40+DuoSet+ELISA/pmc12785530-192-20-21
    Average 93 stars, based on 18 article reviews
    il 12p40 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Induced Pluripotent Stem Cell-Derived Dendritic Cells Provide a Reliable In Vitro Platform for Functional Screening of Immunoregulatory Probiotics"

    Article Title: Induced Pluripotent Stem Cell-Derived Dendritic Cells Provide a Reliable In Vitro Platform for Functional Screening of Immunoregulatory Probiotics

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms27010303

    Cytokine secretion assay of human iPSC-derived immature and mature dendritic cells. Interleukin (IL)-10 and IL-12p40 secretion by human iPSC-derived immature dendritic cells after stimulation with LPS or co-culture with Lactobacillus rhamnosus GG. The “cell only” group refers to iPSC-derived immature dendritic cells without any stimulation. Plates were incubated for 24 h to measure cytokine secretion into the culture medium (*** p < 0.001). Data shown are representative of at least three independent differentiation batches.
    Figure Legend Snippet: Cytokine secretion assay of human iPSC-derived immature and mature dendritic cells. Interleukin (IL)-10 and IL-12p40 secretion by human iPSC-derived immature dendritic cells after stimulation with LPS or co-culture with Lactobacillus rhamnosus GG. The “cell only” group refers to iPSC-derived immature dendritic cells without any stimulation. Plates were incubated for 24 h to measure cytokine secretion into the culture medium (*** p < 0.001). Data shown are representative of at least three independent differentiation batches.

    Techniques Used: Derivative Assay, Co-Culture Assay, Incubation

    Screening candidate probiotic strains for co-culturing with human iPSC-derived dendritic cells. ( a ) Schematic illustration of the co-culture models of human iPSC-derived dendritic cells and probiotic strains. ( b ) IL-6 secretion by human iPSC-derived dendritic cells after co-culturing with different lactic acid bacteria. L. rhamnosus GG was used as the internal control. ( c ) IL-10 secretion by human iPSC-derived dendritic cells after co-culture with different lactic acid bacteria. L. rhamnosus GG was used as the internal control. ( d ). IL-12p40 secretion ratio by human iPSC-derived dendritic cells after co-culturing with different lactic acid bacteria. L. rhamnosus GG was used as the internal control (** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant). Data shown are representative of at least three independent differentiation batches.
    Figure Legend Snippet: Screening candidate probiotic strains for co-culturing with human iPSC-derived dendritic cells. ( a ) Schematic illustration of the co-culture models of human iPSC-derived dendritic cells and probiotic strains. ( b ) IL-6 secretion by human iPSC-derived dendritic cells after co-culturing with different lactic acid bacteria. L. rhamnosus GG was used as the internal control. ( c ) IL-10 secretion by human iPSC-derived dendritic cells after co-culture with different lactic acid bacteria. L. rhamnosus GG was used as the internal control. ( d ). IL-12p40 secretion ratio by human iPSC-derived dendritic cells after co-culturing with different lactic acid bacteria. L. rhamnosus GG was used as the internal control (** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant). Data shown are representative of at least three independent differentiation batches.

    Techniques Used: Derivative Assay, Co-Culture Assay, Bacteria, Control

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: ATP-Induced Inflammation Drives Tissue-Resident Th17 Cells in Metabolically Unhealthy Obesity.
    Article Snippet: The release of cytokines by explants was analyzed in culture supernatants using commercially available IL-1b and IL-6 ELISA kit (BD OptEIA Set; BD Biosciences). .. Human IL-23 levels were analyzed by using Human IL-23 DuoSet ELISA (R&D Systems). .. Human IL-17 levels were analyzed by using Human IL-17A ELISA Ready-SET-Go! (eBioscience).

    Article Title: Assembly-dependent Structure Formation Shapes Human Interleukin-23 versus Interleukin-12 Secretion.
    Article Snippet: .. Concentrations of IL-23 variants, secreted by previously transfected HEK 293 T cells, were analyzed by the human IL-23 DuoSet ELISA (R&D Systems). .. To measure the concentration of IL-12 in HEK supernatants after transfection, the human IL-12 p70 DuoSet ELISA (R&D Systems) was used.

    Article Title: Electrochemical immunosensing of Crohn´s disease biomarkers using diazonium salt grafted - crystalline nanocellulose / carbon nanotubes modified electrodes
    Article Snippet: Anti-IL-12 capture antibody (antiIL12), human IL-12 standard (IL12) and biotin-anti-IL-12 detection antibody (Biotin-anti-IL12) were from the Human IL-12 DuoSet ELISA from R&D Systems (Cat. No. DY1270-05). .. Anti-IL-23 capture antibody (anti-IL23), human IL-23 standard (IL23) and biotin-anti-IL-23 detection antibody (Biotin-anti-IL23) were from the Human IL-23 DuoSet ELISA from R&D Systems (Cat. No. DY1290-05). .. Buffer solutions were 25 mM MES of pH 5.0 prepared from 2-(N-morpholine) ethane-sulfonic acid (Gerbu, Heidelberg, Germany), 100 mM, 50 mM phosphate buffer (PB) of pH 6.0 prepared from Na2HPO4 and NaH2PO4⋅2H2O (SigmaAldrich), and saline phosphate buffer (PBS) consisting of a 100 mM sodium phosphate buffer of pH 7.4 supplemented with 2.0 g NaCl and 50.25 mg KCl (Scharlab) in 250 mL deionized water.

    Article Title: Electrochemical immunosensing of Crohn's disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes.
    Article Snippet: The first dual immunosensor is reported for the determination of IL-12 and IL-23, two relevant biomarkers of Crohn’s disease (CD).. The strategy relies on the selective capture of the targets by the respective antibodies which were covalently immobilized onto SPCEs modified with crystalline nanocellulose (CNC) and multi-walled carbon nanotubes (MWCNTs) followed by conjugation with a detector antibody labelled with poly-HRP-Strept and amperometric transduction using the H2O2/HQ system.. The combination of CNC, a nanomaterial scarcely exploited in immunosensing, with MWCNTs enables the preparation of a novel dual immunosensor for the determination of CD biomarkers in clinical samples, including faeces.

    Transfection:

    Article Title: Assembly-dependent Structure Formation Shapes Human Interleukin-23 versus Interleukin-12 Secretion.
    Article Snippet: .. Concentrations of IL-23 variants, secreted by previously transfected HEK 293 T cells, were analyzed by the human IL-23 DuoSet ELISA (R&D Systems). .. To measure the concentration of IL-12 in HEK supernatants after transfection, the human IL-12 p70 DuoSet ELISA (R&D Systems) was used.



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    Image Search Results


    Cytokine secretion assay of human iPSC-derived immature and mature dendritic cells. Interleukin (IL)-10 and IL-12p40 secretion by human iPSC-derived immature dendritic cells after stimulation with LPS or co-culture with Lactobacillus rhamnosus GG. The “cell only” group refers to iPSC-derived immature dendritic cells without any stimulation. Plates were incubated for 24 h to measure cytokine secretion into the culture medium (*** p < 0.001). Data shown are representative of at least three independent differentiation batches.

    Journal: International Journal of Molecular Sciences

    Article Title: Induced Pluripotent Stem Cell-Derived Dendritic Cells Provide a Reliable In Vitro Platform for Functional Screening of Immunoregulatory Probiotics

    doi: 10.3390/ijms27010303

    Figure Lengend Snippet: Cytokine secretion assay of human iPSC-derived immature and mature dendritic cells. Interleukin (IL)-10 and IL-12p40 secretion by human iPSC-derived immature dendritic cells after stimulation with LPS or co-culture with Lactobacillus rhamnosus GG. The “cell only” group refers to iPSC-derived immature dendritic cells without any stimulation. Plates were incubated for 24 h to measure cytokine secretion into the culture medium (*** p < 0.001). Data shown are representative of at least three independent differentiation batches.

    Article Snippet: The culture supernatants were analyzed using commercially available ELISA kits to measure IL-6 (R&D, #DY206-05), IL-10 (R&D Systems, #DY217B-05), and IL-12p40 (R&D Systems, #DY1240-05), following the manufacturer’s instructions.

    Techniques: Derivative Assay, Co-Culture Assay, Incubation

    Screening candidate probiotic strains for co-culturing with human iPSC-derived dendritic cells. ( a ) Schematic illustration of the co-culture models of human iPSC-derived dendritic cells and probiotic strains. ( b ) IL-6 secretion by human iPSC-derived dendritic cells after co-culturing with different lactic acid bacteria. L. rhamnosus GG was used as the internal control. ( c ) IL-10 secretion by human iPSC-derived dendritic cells after co-culture with different lactic acid bacteria. L. rhamnosus GG was used as the internal control. ( d ). IL-12p40 secretion ratio by human iPSC-derived dendritic cells after co-culturing with different lactic acid bacteria. L. rhamnosus GG was used as the internal control (** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant). Data shown are representative of at least three independent differentiation batches.

    Journal: International Journal of Molecular Sciences

    Article Title: Induced Pluripotent Stem Cell-Derived Dendritic Cells Provide a Reliable In Vitro Platform for Functional Screening of Immunoregulatory Probiotics

    doi: 10.3390/ijms27010303

    Figure Lengend Snippet: Screening candidate probiotic strains for co-culturing with human iPSC-derived dendritic cells. ( a ) Schematic illustration of the co-culture models of human iPSC-derived dendritic cells and probiotic strains. ( b ) IL-6 secretion by human iPSC-derived dendritic cells after co-culturing with different lactic acid bacteria. L. rhamnosus GG was used as the internal control. ( c ) IL-10 secretion by human iPSC-derived dendritic cells after co-culture with different lactic acid bacteria. L. rhamnosus GG was used as the internal control. ( d ). IL-12p40 secretion ratio by human iPSC-derived dendritic cells after co-culturing with different lactic acid bacteria. L. rhamnosus GG was used as the internal control (** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant). Data shown are representative of at least three independent differentiation batches.

    Article Snippet: The culture supernatants were analyzed using commercially available ELISA kits to measure IL-6 (R&D, #DY206-05), IL-10 (R&D Systems, #DY217B-05), and IL-12p40 (R&D Systems, #DY1240-05), following the manufacturer’s instructions.

    Techniques: Derivative Assay, Co-Culture Assay, Bacteria, Control